Vibrio harveyi protease deletion mutant as a live attenuated vaccine candidate against vibriosis and transcriptome profiling following vaccination for Epinephelus fuscoguttatus

Transcriptome data of Epinephelus fuscoguttatus infected by Vibrio vulnificus

Vibriosis disease by Vibrio spp. greatly reduced productivity of aquaculture, such as brown-marbled grouper (Epinephelus fuscoguttatus), which is an economically important fish species in Malaysia. Preventive measures and immediate treatment are critical to reduce the mortality of E. fuscoguttatus from vibriosis. To investigate the molecular mechanisms associated with immune response and host-b...

λ-Red-Recombineering Live Attenuated ΔipaD Shigella dysenteriae from Iranian Isolates as A Candidate of Vaccine

Shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. Although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of Shigella have been reported. Therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. Attenuated live vaccines produced by gene delet...

Construction and characterization of a live, attenuated aroA deletion mutant of Pseudomonas aeruginosa as a candidate intranasal vaccine.

Antibodies to the lipopolysaccharide O antigen of Pseudomonas aeruginosa mediate high-level immunity, but protective epitopes have proven to be poorly immunogenic, while nonprotective or minimally protective O-antigen epitopes often elicit the best immune responses. With the goal of developing a broadly protective P. aeruginosa vaccine, we used a gene replacement system based on the Flp recombi...

Immunogenicity and protective efficacy of a Salmonella Enteritidis sptP mutant as a live attenuated vaccine candidate

BACKGROUND Salmonella enterica serovar Enteritidis (S. Enteritidis) is a highly adaptive pathogen in both humans and animals. As a Salmonella Type III secretion system (T3SS) effector, Salmonella protein tyrosine phosphatase (SptP) is critical for virulence in this genus. To investigate the feasibility of using C50336ΔsptP as a live attenuated oral vaccine in mice, we generated the sptP gene de...

Development of an Attenuated Live Virus Vaccine against Sheep Pox